Journal of Analytical Methods in Chemistry

Research Article

Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro

Table 3

Summary of HPLC method performance: linear equation, linear ranges, and coefficients of phenolic compounds ( = 3).
Phenolic compoundsRetention time (min)SlopeInterceptLinear range (μmol/mL)
Gallic acid7.23 ± 0.071.20 × 109 ± 0.06 × 109−1.00 × 104 ± 0.55 × 1040.01–1.180.999
Catechin15.51 ± 0.111.18 × 108 ± 0.04 × 108−5.17 × 102 ± 0.14 × 1020.01–0.650.999
p-Hydroxybenzoic acid17.61 ± 0.115.78 × 108 ± 0.12 × 1082.96 × 103 ± 1.13 × 1030.01–1.450.999
Vanillic acid22.92 ± 0.127.34 × 108 ± 0.23 × 108−6.89 × 103 ± 1.66 × 1030.01–1.310.999
Caffeic acid23.94 ± 0.121.24 × 109 ± 0.07 × 109−1.50 × 104 ± 0.6 × 1040.01–1.110.999
Ferulaic acid31.73 ± 0.098.15 × 109 ± 0.25 × 109−4.15 × 103 ± 1.66 × 1030.01–0.200.999
Rutin32.34 ± 0.138.26 × 108 ± 0.49 × 1081.99 × 103 ± 0.40 × 1030.01–0.330.999
Benzoic acid34.11 ± 0.149.55 × 107 ± 0.36 × 1073.32 × 103 ± 0.98 × 1030.02–2.050.999
Quercetin45.49 ± 0.159.61 × 108 ± 0.33 × 108−5.11 × 103 ± 1.23 × 1030.01–0.330.999