One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection

Table 1

Calibration curves, LOQ, and LOD of the fourteen investigated AAs.

AAs	Linear regression data			LOQ (𝜇g)	LOD (𝜇g)
	Regressive equation	Test range (mg/mL)	𝑟
Asp	𝑌=509.433𝑋−16.066	0.050–1.000	0.9998	0.017	0.005
Glu	𝑌=449.506𝑋−5.904	0.099–1.986	1.0000	0.040	0.012
Ser	𝑌=642.902𝑋−4.916	0.050–1.000	0.9999	0.020	0.006
Gly	𝑌=890.173𝑋−8.861	0.101–2.016	1.0000	0.015	0.004
Arg	𝑌=398.247𝑋−4.785	0.050–1.005	1.0000	0.040	0.012
Ala	𝑌=796.495𝑋−7.740	0.099–1.987	1.0000	0.030	0.009
Pro	𝑌=659.777𝑋−6.110	0.200–4.006	0.9999	0.020	0.006
Tyr	𝑌=419.329𝑋−3.329	0.035–0.693	0.9999	0.015	0.005
Val	𝑌=595.691𝑋−2.190	0.050–1.002	1.0000	0.015	0.004
Ile	𝑌=555.808𝑋−1.043	0.030–0.607	1.0000	0.010	0.003
Leu	𝑌=560.738𝑋−1.376	0.100–1.993	1.0000	0.013	0.004
Phe	𝑌=455.008𝑋−1.167	0.050–0.990	1.0000	0.030	0.009
Orn	𝑌=619.760𝑋−4.322	0.050–1.010	0.9999	0.012	0.003
Lys	𝑌=869.350𝑋−1.930	0.030–0.605	0.9999	0.010	0.003