One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection

Table 5

Contents of 14 AAs in ten batches of QKLI by two methods.

AAs	013105A		RD^a (%)	110101A		RD^a (%)	115202A		RD^a (%)	210204A		RD^a (%)	111103A		RD^a (%)
	a	b		a	b		a	b		a	b		a	b
Gly	—	1.039	—	—	1.028	—	—	0.997	—	—	0.983	—	—	1.046	—
Asp	0.273	0.269	1.29	0.324	0.321	0.81	0.315	0.312	0.88	0.338	0.336	0.70	0.339	0.337	0.69
Glu	1.055	1.074	−1.74	1.008	1.026	−1.72	0.922	0.937	−1.67	0.954	0.970	−1.69	0.996	1.013	−1.71
Ser	0.284	0.284	−0.04	0.296	0.296	−0.04	0.288	0.288	−0.04	0.254	0.254	−0.03	0.273	0.273	−0.04
Arg	0.424	0.429	−1.19	0.434	0.440	−1.20	0.711	0.720	−1.31	0.661	0.670	−1.30	0.611	0.619	−1.28
Ala	1.200	1.211	−0.86	1.133	1.142	−0.81	1.074	1.082	−0.76	1.078	1.087	−0.77	1.180	1.190	−0.85
Pro	2.284	2.254	1.34	2.471	2.440	1.26	2.179	2.149	1.38	2.357	2.326	1.31	2.832	2.800	1.15
Tyr	0.234	0.233	0.57	0.270	0.269	0.39	0.251	0.250	0.48	0.196	0.194	0.83	0.237	0.236	0.56
Val	0.537	0.542	−0.91	0.511	0.515	−0.83	0.483	0.486	−0.73	0.506	0.510	−0.81	0.555	0.560	−0.95
Ile	0.264	0.263	0.32	0.249	0.248	0.49	0.232	0.231	0.72	0.246	0.245	0.53	0.273	0.272	0.23
Leu	1.209	1.212	−0.24	1.124	1.126	−0.15	1.075	1.075	−0.08	1.070	1.071	−0.08	1.185	1.188	−0.22
Trp	0.408	0.403	1.20	0.377	0.372	1.35	0.368	0.362	1.40	0.378	0.373	1.34	0.404	0.399	1.22
Orn	0.353	0.355	−0.58	0.322	0.324	−0.63	0.323	0.325	−0.63	0.314	0.316	−0.64	0.328	0.330	−0.62
Lys	0.245	0.254	−3.50	0.218	0.226	−3.41	0.234	0.243	−3.46	0.228	0.236	−3.44	0.244	0.253	−3.50
AAs	115203A		RD^a (%)	210401A		RD^a (%)	012602A		RD^a (%)	210301A		RD^a (%)	115702A		RD^a (%)
	a	b		a	b		a	b		a	b		a	b
Gly	—	1.077	—	—	1.115	—	—	1.145	—	—	1.125	—	—	1.138	—
Asp	0.359	0.357	0.55	0.356	0.354	0.57	0.375	0.373	0.46	0.372	0.370	0.48	0.397	0.395	0.34
Glu	1.016	1.034	−1.72	0.978	0.995	−1.70	0.966	0.983	−1.70	0.997	1.015	−1.71	1.094	1.114	−1.76
Ser	0.332	0.332	−0.06	0.301	0.301	−0.05	0.275	0.275	−0.04	0.279	0.279	−0.04	0.324	0.324	−0.05
Arg	0.821	0.832	−1.34	0.659	0.668	−1.30	0.596	0.604	−1.28	0.483	0.489	−1.23	0.576	0.583	−1.27
Ala	1.167	1.177	−0.84	1.199	1.209	−0.86	1.233	1.244	−0.88	1.184	1.194	−0.85	1.254	1.265	−0.90
Pro	2.367	2.337	1.30	2.489	2.458	1.26	2.919	2.886	1.13	2.251	2.221	1.35	2.225	2.195	1.36
Tyr	0.257	0.256	0.45	0.217	0.216	0.68	0.289	0.288	0.32	0.213	0.211	0.71	0.247	0.246	0.50
Val	0.525	0.529	−0.87	0.538	0.543	−0.91	0.530	0.535	−0.88	0.533	0.537	−0.89	0.566	0.571	−0.98
Ile	0.251	0.250	0.47	0.259	0.258	0.37	0.258	0.257	0.39	0.263	0.262	0.33	0.279	0.278	0.17
Leu	1.161	1.163	−0.19	1.166	1.168	−0.19	1.205	1.208	−0.24	1.167	1.170	−0.20	1.243	1.246	−0.27
Trp	0.390	0.385	1.29	0.393	0.388	1.27	0.408	0.403	1.20	0.408	0.403	1.20	0.431	0.426	1.11
Orn	0.362	0.365	−0.56	0.349	0.351	−0.58	0.366	0.368	−0.56	0.380	0.382	−0.54	0.382	0.384	−0.53
Lys	0.257	0.267	−3.53	0.250	0.259	−3.51	0.257	0.267	−3.53	0.265	0.275	−3.56	0.268	0.278	−3.56

aRelative deviation = (a − b)/b.
a: the content were calculated by One for M method; b: the content were determined by the traditional calibration equation method.