Journal of Analytical Methods in Chemistry / 2014 / Article / Tab 2

Research Article

Quantification of Caffeoylquinic Acids in Coffee Brews by HPLC-DAD

Table 2

Validation parameters for CQAs analysis by HPLC-DAD.

Validation
parameters
Concentration
levelsa
3-CQA4-CQA5-CQA

Linearity range (mg/L)2–4001–2001–200
()b0.9990.9990.999
Sensitivityc116292122162111337
Limit of detection (mg/L)d0.370.390.18
Limit of quantification (mg/L)d1.241.290.58
Intraday precision (%CV)C11.060.950.36
C21.060.800.54
C30.310.170.24
Interday precision (%CV)C10.810.540.36
C20.460.680.23
C31.111.410.32

Concentration of each compound in standard solutions was as follows. C1: 3-CQA (40 mg/L), 4-CQA (20 mg/L), and 5-CQA (20 mg/L); C2: 3-CQA (160 mg/L), 4-CQA (80 mg/L), and 5-CQA (80 mg/L); C3: 3-CQA (320 mg/L), 4-CQA (160 mg/L), and 5-CQA (160 mg/L).
coefficient of determination, number of calibration curve standards.
cSensitivity was expressed as the slope of the calibration curves.
dCalculated from the signal to noise ratio of 3 (LOD) and 10 (LOQ).

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