Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics

Figure 2

Starting with a complex body fluid sample a specific peptide or protein can be enriched by means of immunoaffinity capture. Whereas a subfraction of the sample is analyzed in an SPE approach, profiling compounds that match the binding capabilities of the sorbent (a), affinity enrichment results in “clean” mass spectra (proteolytic peptide in (b), intact protein in (c)).