Research Article
Response Surface Optimization of a Rapid Ultrasound-Assisted Extraction Method for Simultaneous Determination of Tetracycline Antibiotics in Manure
Table 2
Box-Behnken design with actual/coded values and results of tetracycline antibiotics recovery.
| | | pH | volume (mL) | temperature (°C) | recovery (%) |
| | 1 | 6 (−1) | 30 (−1) | 30 (0) | 72.20 | | 2 | 8 (+1) | 30 (−1) | 30 (0) | 74.49 | | 3 | 6 (−1) | 50 (+1) | 30 (0) | 78.65 | | 4 | 8 (+1) | 50 (+1) | 30 (0) | 83.15 | | 5 | 6 (−1) | 40 (0) | 20 (−1) | 77.22 | | 6 | 8 (+1) | 40 (0) | 20 (−1) | 82.05 | | 7 | 6 (−1) | 40 (0) | 40 (+1) | 88.63 | | 8 | 8 (+1) | 40 (0) | 40 (+1) | 88.23 | | 9 | 7 (0) | 30 (−1) | 20 (−1) | 76.69 | | 10 | 7 (0) | 50 (+1) | 20 (−1) | 85.70 | | 11 | 7 (0) | 30 (−1) | 40 (+1) | 83.48 | | 12 | 7 (0) | 50 (+1) | 40 (+1) | 92.26 | | 13 | 7 (0) | 40 (0) | 30 (0) | 80.59 | | 14 | 7 (0) | 40 (0) | 30 (0) | 81.70 | | 15 | 7 (0) | 40 (0) | 30 (0) | 80.58 |
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