Quantitative Determination of Lercanidipine Enantiomers in Commercial Formulations by Capillary Electrophoresis

Table 5

Linearity, LOD and LOQ, precision, and accuracy of the method for LER enantiomer analysis.

Limits	(S)-LER			(R)-LER
LOD (g/mL)	0.8			0.6
LOQ (g/mL)	2.4			1.9
Slope (RSD)	5.8			1.0
Intercept (RSD)	12.4			30.5
Linearity
Linear equation
Correlation coefficient ()	0,998			0,995
Precision and accuracy
Nominal concentration (g/mL)	12.5	50	100	12.5	50	100
Within-day ()
Analyzed concentration (g/mL)	12.4	49.8	101.1	12.4	51.2	100.1
Precision (RSD, %)^b	3.4	4.4	3.1	4.5	3.7	3.2
Accuracy (, %)^c	−0.8	−0.4	1.1	−0.8	2.4	0.1
Between-day ()
Analyzed concentration (g/mL)	12.3	51.7	98.6	12.3	52.1	98.4
Precision (RSD, %)^b	3.6	4.3	4.0	4.3	4.0	3.8
Accuracy (, %)^c	−1.6	3.4	−1.4	−1.6	4.2	−1.6

Calibration graphs were prepared in triplicate () for concentrations of 12.5, 25, 50, 75, and 100 g/mL; , where is the peak height of LER enantiomers, is the slope, is the intercept, and is the concentration of the measured solution in g/mL; : number of determinations; ^bRSD (%): relative standard deviation expressed as percentage; (%): relative error expressed as percentage; : number of days.