Research Article

Analysis of Reaction between -Lipoic Acid and 2-Chloro-1-methylquinolinium Tetrafluoroborate Used as a Precolumn Derivatization Technique in Chromatographic Determination of -Lipoic Acid

Table 1

Features of the developed methodology.

ParameterStudied rangeSelected value

Molar ratio LA : NaBH41 : 25–1 : 1001 : 50
pH of reduction7–109,5
Temperature of reduction/time of heating 60°C
0–15 min
6 min
pH of medium of reaction DHLA-CMQT4.5–106
Excess of CMQT in ratio to DHLA1 : 1–1 : 51 : 5