Journal of Analytical Methods in Chemistry / 2015 / Article / Tab 2

Research Article

New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant

Table 2

Metabolite composition of tea flush of cultivars DT1 and TRI 2025 determined using two methods of sampling with fresh tea flush (conventional procedure) and freeze-dried tea flush (new procedure).

MetabolitesDT1 (mg g−1)TRI 2025 (mg g−1)
FreshFreeze-driedLSDCVFreshFreeze-driedLSDCV

TPP††266.49 ± 2.79282.27 ± 3.773.561.21266.33 ± 2.72278.07 ± 3.543.391.16
EC15.88 ± 0.2919.27 ± 0.270.481.596.27 ± 0.547.47 ± 0.410.826.9
ECg37.65 ± 1.2241.43 ± 0.531.632.3825.30 ± 1.4530.71 ± 0.491.873.86
EGC33.99 ± 0.9142.21 ± 0.561.311.9917.54 ± 1.0620.01 ± 0.921.725.28
EGCg84.00 ± 5.1593.61 ± 1.146.454.20104.17 ± 3.22128.95 ± 2.334.872.41
Caffeine35.31 ± 1.3638.67 ± 0.331.712.6734.52 ± 0.1341.71 ± 0.590.741.12
Theobromine2.17 ± 0.152.25 ± 0.02NS0.184.904.62 ± 0.325.89 ± 0.170.454.91
Gallic acid0.29 ± 0.010.55 ± 0.030.034.260.31 ± 0.010.83 ± 0.010.021.59

Means ± SD (), : probability, significant at 5% level of probability, significant at 1% level of probability, NS: not significantly different, CV: coefficient of variance, LSD: Least Significant Difference, ††determined by ISO-14052-1 Spectrophotometric method, determined by ISO-14052-2 HPLC method, and TPP: total polyphenols.