New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant
Table 2
Metabolite composition of tea flush of cultivars DT1 and TRI 2025 determined using two methods of sampling with fresh tea flush (conventional procedure) and freeze-dried tea flush (new procedure).
Metabolites
DT1 (mg g−1)
TRI 2025 (mg g−1)
Fresh
Freeze-dried
LSD
CV
Fresh
Freeze-dried
LSD
CV
TPP††
266.49 ± 2.79
282.27 ± 3.77
3.56
1.21
266.33 ± 2.72
278.07 ± 3.54
3.39
1.16
EC†
15.88 ± 0.29
19.27 ± 0.27
0.48
1.59
6.27 ± 0.54
7.47 ± 0.41
0.82
6.9
ECg†
37.65 ± 1.22
41.43 ± 0.53
1.63
2.38
25.30 ± 1.45
30.71 ± 0.49
1.87
3.86
EGC†
33.99 ± 0.91
42.21 ± 0.56
1.31
1.99
17.54 ± 1.06
20.01 ± 0.92
1.72
5.28
EGCg†
84.00 ± 5.15
93.61 ± 1.14
6.45
4.20
104.17 ± 3.22
128.95 ± 2.33
4.87
2.41
Caffeine†
35.31 ± 1.36
38.67 ± 0.33
1.71
2.67
34.52 ± 0.13
41.71 ± 0.59
0.74
1.12
Theobromine†
2.17 ± 0.15
2.25 ± 0.02
NS
0.18
4.90
4.62 ± 0.32
5.89 ± 0.17
0.45
4.91
Gallic acid†
0.29 ± 0.01
0.55 ± 0.03
0.03
4.26
0.31 ± 0.01
0.83 ± 0.01
0.02
1.59
Means ± SD (), : probability, significant at 5% level of probability, significant at 1% level of probability, NS: not significantly different, CV: coefficient of variance, LSD: Least Significant Difference, ††determined by ISO-14052-1 Spectrophotometric method, †determined by ISO-14052-2 HPLC method, and TPP: total polyphenols.
