Research Article
Simultaneous Determination of Six Active Compounds in Yixin Badiranjibuya Granules, a Traditional Chinese Medicine, by RP-HPLC-UV Method
Table 1
The different separation system (see Figure
2).
| | Chromatograms | Column | Elution programs |
| | (A) | Shim-pack ODS C18 column | 0–30 min, isocratic 13% (v/v) acetonitrile
30–55 min, linear gradient 13%–22% (v/v) acetonitrile
55–70 min, linear gradient 22%–40% (v/v) acetonitrile |
| | (B) | Merck Purospher STAR C18 column | 0–30 min, isocratic 13% (v/v) acetonitrile
30–55 min, linear gradient 13%–22% (v/v) acetonitrile
55–70 min, linear gradient 22%–40% (v/v) acetonitrile |
| | (C) | Merck Purospher STAR C18 column | 0–30 min, isocratic 18% (v/v) acetonitrile
30–60 min, linear gradient 18%–30% (v/v) acetonitrile
60–70 min, isocratic 30% (v/v) acetonitrile |
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