Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang

Table 3

Precision and repeatability for the assay of the 10 major compounds.

Compounds	Concentration (ng/mL)	Precision ()		Repeatability ()
		Found	RSD (%)	Found	RSD (%)
Tetrandrine	52.08	51.49 ± 0.46	0.89	53.52 ± 1.16	2.15
Fangchinoline	58.77	57.56 ± 0.49	0.84	55.87 ± 0.85	1.52
Atractylenolide I	11.77	11.51 ± 0.26	2.27	12.26 ± 0.26	2.15
Atractylenolide III	17.82	17.47 ± 0.14	0.78	21.60 ± 0.58	2.68
Calycosin-7-O-β-D-glucoside	317.96	317.81 ± 0.58	0.18	145.27 ± 1.86	1.28
Glycyrrhizin	1003.78	1003.86 ± 1.18	0.12	1661.02 ± 34.97	2.11
Liquiritin	3960.00	3941.00 ± 46.23	1.17	3303.74 ± 24.75	0.75
Isoliquiritin	972.02	974.07 ± 1.20	0.12	695.73 ± 8.31	1.19
Liquiritigenin	67.78	68.90 ± 0.89	1.29	44.50 ± 0.83	1.86
Isoliquiritigenin	19.51	18.94 ± 0.48	2.53	3.11 ± 0.06	2.03