Table 3: Precision and repeatability for the assay of the 10 major compounds.

CompoundsConcentration (ng/mL)Precision ()Repeatability ()
FoundRSD (%)FoundRSD (%)

Tetrandrine52.0851.49 ± 0.460.8953.52 ± 1.162.15
Fangchinoline58.7757.56 ± 0.490.8455.87 ± 0.851.52
Atractylenolide I11.7711.51 ± 0.262.2712.26 ± 0.262.15
Atractylenolide III17.8217.47 ± 0.140.7821.60 ± 0.582.68
Calycosin-7-O-β-D-glucoside317.96317.81 ± 0.580.18145.27 ± 1.861.28
Glycyrrhizin1003.781003.86 ± 1.180.121661.02 ± 34.972.11
Liquiritin3960.003941.00 ± 46.231.173303.74 ± 24.750.75
Isoliquiritin972.02974.07 ± 1.200.12695.73 ± 8.311.19
Liquiritigenin67.7868.90 ± 0.891.2944.50 ± 0.831.86
Isoliquiritigenin19.5118.94 ± 0.482.533.11 ± 0.062.03