Table 4: Recovery of the 10 major compounds in Fangji Huangqi Tang ().

CompoundsOriginal amount (ng)Spiked amount (ng)Detected amount (ng)Recovery (%)RSD (%)

Tetrandrine1304.50 1045.00 2343.50 ± 2.5099.24 0.91
1306.00 2601.00 ± 20.50
1567.50 2857.00 ± 18.00

Fangchinoline1392.50 1111.50 2480.00 ± 27.5099.271.08
1389.50 2782.50 ± 5.00
1667.00 3057.00 ± 21.50

Atractylenolide I294.00 232.00 529.00 ± 2.50101.11.40
290.00 584.00 ± 5.50
348.00 647.50 ± 0.50

Atractylenolide III540.00 431.00 965.50 ± 10.0099.881.78
538.50 1079.00 ± 1.50
646.50 1192.00 ± 19.50

Calycosin-7-O-β-D-glucoside3622.00 2897.00 6512.50 ± 6.0099.590.29
3621.00 7203.00 ± 14.50
4345.50 7973.00 ± 79.00

Glycyrrhizin41970.00 33575.00 75216.00 ± 145.0098.740.78
41969.00 83537.50 ± 362.00
50362.50 91399.50 ± 501.00

Liquiritin82593.50 66074.00 148436.50 ± 254.5099.180.96
82592.50 163987.50 ± 1503.50
99111.00 181061.00 ± 627.50

Isoliquiritin17393.50 13914.50 31167.50 ± 95.5098.840.47
17393.00 34595.00 ± 80.00
20871.50 37982.00 ± 52.00

Liquiritigenin1112.50 890.50 1991.00 ± 9.5098.511.18
1113.00 2209.00 ± 14.50
1335.50 2426.00 ± 18.00

Isoliquiritigenin79.00 63.00 141.50 ± 1.0099.551.09
78.50 156.50 ± 0.50
94.50 173.50 ± 0.50