Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang

Table 4

Recovery of the 10 major compounds in Fangji Huangqi Tang ().

Compounds	Original amount (ng)	Spiked amount (ng)	Detected amount (ng)	Recovery (%)	RSD (%)
Tetrandrine	1304.50	1045.00	2343.50 ± 2.50	99.24	0.91
		1306.00	2601.00 ± 20.50
		1567.50	2857.00 ± 18.00
Fangchinoline	1392.50	1111.50	2480.00 ± 27.50	99.27	1.08
		1389.50	2782.50 ± 5.00
		1667.00	3057.00 ± 21.50
Atractylenolide I	294.00	232.00	529.00 ± 2.50	101.1	1.40
		290.00	584.00 ± 5.50
		348.00	647.50 ± 0.50
Atractylenolide III	540.00	431.00	965.50 ± 10.00	99.88	1.78
		538.50	1079.00 ± 1.50
		646.50	1192.00 ± 19.50
Calycosin-7-O-β-D-glucoside	3622.00	2897.00	6512.50 ± 6.00	99.59	0.29
		3621.00	7203.00 ± 14.50
		4345.50	7973.00 ± 79.00
Glycyrrhizin	41970.00	33575.00	75216.00 ± 145.00	98.74	0.78
		41969.00	83537.50 ± 362.00
		50362.50	91399.50 ± 501.00
Liquiritin	82593.50	66074.00	148436.50 ± 254.50	99.18	0.96
		82592.50	163987.50 ± 1503.50
		99111.00	181061.00 ± 627.50
Isoliquiritin	17393.50	13914.50	31167.50 ± 95.50	98.84	0.47
		17393.00	34595.00 ± 80.00
		20871.50	37982.00 ± 52.00
Liquiritigenin	1112.50	890.50	1991.00 ± 9.50	98.51	1.18
		1113.00	2209.00 ± 14.50
		1335.50	2426.00 ± 18.00
Isoliquiritigenin	79.00	63.00	141.50 ± 1.00	99.55	1.09
		78.50	156.50 ± 0.50
		94.50	173.50 ± 0.50