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Journal of Analytical Methods in Chemistry
Volume 2016, Article ID 3827832, 8 pages
http://dx.doi.org/10.1155/2016/3827832
Research Article

Determination of Total Apigenin in Herbs by Micellar Electrokinetic Chromatography with UV Detection

Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, 163 Pomorska Street, 90-236 Łódź, Poland

Received 10 May 2016; Revised 7 June 2016; Accepted 8 June 2016

Academic Editor: Chih-Ching Huang

Copyright © 2016 Rafał Głowacki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Apigenin is a naturally occurring plant flavone that exhibits strong antioxidant, anti-inflammatory, and antitumor properties. A MEKC-UV based method was developed for the determination of total apigenin in selected herbs. Application of pseudostationary phase in the form of SDS micelles resulted in great repeatability of retention times and peak areas. A buffer solution consisting of 30 mmol/L sodium borate (pH 10.2), 10% acetonitrile, and 10 mmol/L sodium dodecyl sulfate was found to be the most suitable BGE for the separation. The method was validated and calibrated for total apigenin in the range of 1.0–100 μmol/L (). The limits of detection and quantification were 0.48 μmol/L and 0.92 μmol/L, respectively. This precise and robust method was successfully applied to the analysis of plant samples for total apigenin content.