Table of Contents Author Guidelines Submit a Manuscript
Journal of Analytical Methods in Chemistry
Volume 2016, Article ID 4306838, 10 pages
http://dx.doi.org/10.1155/2016/4306838
Research Article

Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity

1Department of Pharmacy, Xi’an Medical College, Xi’an 710021, China
2Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, China
3Beijing Research Center of Agricultural Standards and Testing, Beijing 100097, China

Received 12 June 2016; Revised 25 August 2016; Accepted 5 September 2016

Academic Editor: Jaroon Jakmunee

Copyright © 2016 Lingzhi Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: UV-Vis absorption responses of PPase activity assays based on colorimetric method of molybdenum blue in the absence (red line) and the presence of 200 μM (blue line) or 500 μM DHC (black line), respectively. The reaction conditions are shown in 2.4 of the experimental section. Inset: the corresponding photos of PPase activity assays based on colorimetric method of molybdenum blue in the absence (left) and the presence of 200 μM Cu2+ (middle) or 500 μM DHC (right).

Figure S2: (A) Fluorescence responses of DHC (500 μM) (curve a), Cu2+(200 μM) - DHC (500 μM) complex with NH3Cl (curve b to j: 0, 10, 30, 50, 100, 200, 400, 600, 800 μM) in HEPES aqueous buffer (10 mM, pH 6.5). (B) Fluorescence responses of DHC (500 μM) (curve a), Cu2+ (200 μM) - DHC (500 μM) complex with EDTA (curve b to j: 0, 5, 10, 30, 50, 70, 100, 200, 400 μM) in HEPES aqueous buffer (10 mM, pH 6.5). Other reaction conditions are the same as Figure 1.

Figure S3: Fluorescence responses of DHC (500 μM) (curve a), Cu2+(200 μM) - DHC (500 μM) complex with GSH (A, curve b to g: 0, 30, 50, 100, 200, 400 μM), Cys (B, curve b to i: 0, 10, 30, 50, 100, 200, 400, 800 μM), Hcy (C, curve b to j: 0, 10, 30, 50, 100, 200, 400, 800 μM) in HEPES aqueous buffer (10 mM, pH 6.5). Other reaction conditions are the same as Figure 1.

  1. Supplementary Material