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Journal of Analytical Methods in Chemistry
Volume 2016, Article ID 7528297, 15 pages
Research Article

Model Analytical Development for Physical, Chemical, and Biological Characterization of Momordica charantia Vegetable Drug

1Department of Pharmaceutical Sciences, Postgraduate Program in Pharmaceutical Sciences, Federal University of Pernambuco, University City, Artur de Sá Avenue, 50740-521 Recife, PE, Brazil
2Unified Laboratories of Pharmaceutical Development and Assays, Department of Pharmaceutical Sciences, Federal University of Paraíba, Campus I, University City, 58059-970 João Pessoa, PB, Brazil

Received 3 February 2016; Revised 9 May 2016; Accepted 15 June 2016

Academic Editor: Eduardo Dellacassa

Copyright © 2016 Deysiane Oliveira Brandão et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Momordica charantia is a species cultivated throughout the world and widely used in folk medicine, and its medicinal benefits are well documented, especially its pharmacological properties, including antimicrobial activities. Analytical methods have been used to aid in the characterization of compounds derived from plant drug extracts and their products. This paper developed a methodological model to evaluate the integrity of the vegetable drug M. charantia in different particle sizes, using different analytical methods. M. charantia was collected in the semiarid region of Paraíba, Brazil. The herbal medicine raw material derived from the leaves and fruits in different particle sizes was analyzed using thermoanalytical techniques as thermogravimetry (TG) and differential thermal analysis (DTA), pyrolysis coupled to gas chromatography/mass spectrometry (PYR-GC/MS), and nuclear magnetic resonance (1H NMR), in addition to the determination of antimicrobial activity. The different particle surface area among the samples was differentiated by the techniques. DTA and TG were used for assessing thermal and kinetic parameters and PYR-GC/MS was used for degradation products chromatographic identification through the pyrograms. The infusions obtained from the fruit and leaves of Momordica charantia presented antimicrobial activity.