Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

<div>Major steps for F-S method. (a) Sample being centrifuged at 40000 rpm. (b) Gradient sucrose solution being frozen at −80°C; (c) ice bar being unloaded from centrifuge tube into dry ice. (d) Frozen bar being sliced. (e) Frozen bar fixed on stage. (f) Status and shape of freshly sliced ice pieces.</div>

Journal of Analytical Methods in Chemistry

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Figure 1

Figure 1: Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry 

Figure 1 | Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry 