Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Table 1

Molecular weight of each subunit of complex .

Subunit	MW
Alpha (α)	59,754	√
Beta (β)	56,563	√
Gamma (γ)	32,997	√
	28,910	✕
	24,817	√
OSCP	23,277	✕
	18,360	√
Delta (δ)	17,490	✕
	14,277/14638/14693	√
F6	12,588	√
	11,387	√
	10,787	√
8 (A6L)	7,992	√
	7,802	√
Epsilon (ε)	5,648	√

Data cited from human mitochondrial protein database was developed by the National Institute of Standards and Technology. √ indicates being identifiable, and ✕ indicates being not identifiable.