UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃ and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

Table 2

System suitability test parameters.

Parameters	Analyte/technique
	HPLC		UHPLC
	Vitamin D3	MK-7	Vitamin D3	MK-7
Retention time ( in min)	5.11	14.49	1.26	3.64
Tailing factor ()	1.11	1.12	0.94	0.98
Resolution (Rs)	—	15.92	—	27.61
Selectivity factor ()	—	6.83	—	4.61
Theoretical plates ()	5867	9684	23289	28521
% RSD of retention time ()	0.003	0.002	0.002	0.004