Research Article
UHPLC-PDA Assay for Simultaneous Determination of Vitamin D3 and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation
Table 3
Statistical evaluation of regression data of vitamin D3 and MK-7 by HPLC and UHPLC.
| Parameters | Analyte/technique | HPLC | UHPLC | Vitamin D3 | MK-7 | Vitamin D3 | MK-7 |
| Linearity range (μg mL−1) | 5–100 | 5–100 | 5–100 | 5–100 | Slope | 1998606 | 136363 | 0.5854 | 0.5854 | Intercept | 5725724 | 70561.4 | 134.52 | 104.52 | Standard error of slope | 92902 | 6808 | 3.6 × 10−2 | 4.43 × 10−2 | Standard error of intercept | 469133 | 34377 | 1.181 | 1.901 | Coefficient of determination () | 0.9984 | 0.9981 | 0.9984 | 0.9981 | Limit of detection (μg mL−1) | 0.24 | 0.27 | 0.16 | 0.18 | Limit of quantification (μg mL−1) | 0.72 | 0.81 | 0.48 | 0.54 |
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