Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

Table 1

Assay validation sheet of the proposed methods for the determination of Oxytetracycline HCl and Flunixin Meglumine.

Parameter	Dual wavelength		First derivative		Ratio difference		Derivative ratio
	OXY	FLU	OXY	FLU	OXY	FLU	OXY	FLU
Accuracy; (mean ± RSD)
Repeatability precision
Intermediate precision
Linearity
Slope	0.0059	0.0059	0.0038	0.0023	0.7847	0.3142	1.3421	0.1583
Intercept	0.0139	0.0216	0.0072	0.0088	2.1503	0.7429	2.1807	0.5127
Correlation coefficient (r)	0.9999	0.9999	0.9998	0.9999	0.9999	0.9999	0.9998	0.9998
Range	7–60 µg/mL	5–60 µg/mL	5–60 µg/mL	5–60 µg/mL	5–60 µg/mL	5–60 µg/mL	5–60 µg/mL	5–60 µg/mL

The intraday (n = 3), RSD of three concentrations of 10, 30, and 60 µg/mL for OXY and of 10, 30, and 60 µg/mL for FLU repeated three times within the day. The interday (n = 3), RSD of three concentrations of 10, 30, and 60 µg/mL for OXY and of 10, 30, and 60 µg/mL for FLU repeated three times in three successive days.