LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

Table 1

The number of metabolic features detected in different aqueous humor extracts.

Extraction solutions	Number of metabolic features detected	⁢The % of metabolic features measured with given reproducibility
		CV < 30%	CV < 20%
Acetonitrile	1102	92.4%	87.6%
Acetone	1200	91.2%	85.4%
Methanol/acetone/acetonitrile	1196	91.0%	85.9%
Methanol/ethanol	1165	92.8%	87.9%

In mixed solutions the solvents were used in equal proportions. The numbers of metabolic features detected do not include features present in the blank extraction. Presented results include data from both ion modes.