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Journal of Analytical Methods in Chemistry
Volume 2017 (2017), Article ID 6749087, 7 pages
https://doi.org/10.1155/2017/6749087
Research Article

Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection

1Department of Pharmacy, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, China
2Department of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
3Department of Oncology, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, China

Correspondence should be addressed to Bao-xia Fang; moc.361@501118-xbf

Received 21 October 2016; Accepted 19 December 2016; Published 11 January 2017

Academic Editor: Chih-Ching Huang

Copyright © 2017 Fu-chao Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatographic separation was achieved on a Phenomenex C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH2PO4 buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0). Flow rate was 1.0 mL/min with a column temperature of 30°C. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits. The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use.