Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection

Table 1

System suitability parameters for DEX, OND, GRA, TRO, and AZA.

Analytical parameter	DEX	OND	GRA	TRO	AZA
Detection wavelength (nm)	241	310	302	285	302
Retention time (min)	10.72	8.12	5.97	7.10	4.55
Theoretical plate (mean ± SD)
Linear range (mg/L)	1.0–100.0	2.0–80.0	1.2–36.0	1.0–50.0	2.0–100.0
Linear equation
Correlation coefficient ()	0.9992	0.9999	0.9997	0.9995	0.9998
Quantification limit (mg/L)	0.1	0.3	0.18	0.15	0.6
Detection limit (mg/L)	0.04	0.1	0.06	0.05	0.2