The Evidence of Cooperative Binding of a Ligand to G4 DNA

Table 1

Intrinsic binding constants calculated for simulated ligand G4 DNA equilibrium systems.

	Data matrix	,; theoretical; model	,; LS calc.; parameters	lof; %	%	%
Identical sites
Cooperative binding without SI		,; 2.0	,; 1.0	0.2	18.4	1.0
Anticooperative binding without SI		,; 0.3	,; 1.0	≈0	≈0	≈0
Cooperative binding with SI		,; 2.0	,; 1.0	0.8	19.4	2.3
Anticooperative binding with SI		,; 0.3	,; 1.0	≈0	≈0	≈0
Different sites
Binding without SI		,; 1	,; 0.58	≈0	≈0	≈0

is the rank of full data matrix ; is the rank of data matrix part covering spectral window of a ligand; SI is spectral interference; parameter is fixed; lof < 10⁻⁴≈ 0; = 5 μM.