The Evidence of Cooperative Binding of a Ligand to G4 DNA

Table 2

Intrinsic binding constants for TMPyP4 binding to G4 oligomer calculated from experimental UV-Vis titrations (pH 7.1, = 25°C).

A model	Data matrix	,; LS calculated parameters	lof; %	%	rank; ()	rank; ()	rank; ()
Stepwise complex formation		,; 1.0	0.25	≈0	3 ()	2 ()	—
Cooperative binding		,; 0.43	0.25	≈0	—	—	1 ()

is the rank of full data matrix ; is the rank of data matrix part covering spectral window of a ligand 340–540 nm.