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Journal of Analytical Methods in Chemistry
Volume 2017 (2017), Article ID 9086467, 7 pages
Research Article

Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles

1Facultad de Ciencias Biológicas, Laboratorio de Nanotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba s/n, 66455 San Nicolás de los Garza, NL, Mexico
2Univ Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEP-UMR 5007, 69622 Lyon, France
3Facultad de Medicina, Departamento de Química Analítica, Universidad Autónoma de Nuevo León, Av. Fco. I. Madero y Dr. E. Aguirre Pequeño s/n, 64460 Monterrey, NL, Mexico

Correspondence should be addressed to Sergio Arturo Galindo-Rodríguez; xm.moc.oohay@dorgas

Received 18 May 2017; Revised 11 September 2017; Accepted 13 September 2017; Published 22 November 2017

Academic Editor: Anna V. Queralt

Copyright © 2017 Claudia Janeth Martínez-Rivas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The interest on plants has been focalized due to their biological activities. Extracts or fractions from plants in biodegradable polymeric nanoparticles (NP) provide many advantages on application studies. The encapsulation of the extract or fraction in NP is determined for the establishment of the test dose. HPLC method is an alternative to calculate this parameter. An analytical method based on HPLC for quantification of a hexane fraction from L. frutescens was developed and validated according to ICH. Different concentrations of the hexane fraction from leaves (HFL) were prepared (100–600 μg/mL). Linearity, limit of detection, limit of quantification, and intra- and interday precision parameters were determined. HFL was encapsulated by nanoprecipitation technique and analyzed by HPLC for quantitative aspect. The method was linear and precise for the quantification of the HFL components. NP size was 190 nm with homogeneous size distribution. Through validation method, it was determined that the encapsulation of components (), (), (), and () was 44, 74, 86, and 97%, respectively. A simple, repeatable, and reproducible methodology was developed for the propose of quantifying the components of a vegetable material loaded in NP, using as a model the hexane fraction of L. frutescens leaves.