Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets

Table 2

Results obtained by the HPLC-UV and UPLC-UV methods for the simultaneous determination of CANA and MET in their binary mixture.

Parameters	HPLC		UPLC
	CANA	MET	CANA	MET
Linearity range (µg·mL^−1)	1–50	0.5–90	0.1–50	0.25–100
Slope	0.9102	0.9319	0.8821	1.0795
Intercept	0.3367	0.255	0.1499	0.5372
Correlation coefficient	0.9999	0.9999	0.9999	0.9999
Accuracy (mean ± SD)	99.81 ± 0.73	99.37 ± 0.54	99.47 ± 1.034	99.73 ± 0.89
LOD	0.7154	0.9327	0.463	0.614
LOQ	2.1075	2.8263	1.406	1.862
Precision (% RSD) Repeatability	1.31	1.47	0.48	0.71
Interday precision (reproducibility)	1.75	0.90	0.89	1.85
Specificity (mean ± SD)	99.60 ± 0.33	100.38 ± 1.20	99.89 ± 1.39	99.09 ± 1.31