A Standardized Approach to Quantitative Analysis of Nicotine in e-Liquids Based on Peak Purity Criteria Using High-Performance Liquid Chromatography

Table 3

HPLC gradient program.

Pump program
Time (min)	A (%)	B (%)	C (%)	D (%)
0	60	26	14	0
4	60	26	14	0
4.1	0	0	0	100
7	0	0	0	100
7.1	60	26	14	0
12	60	26	14	0