A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium

Table 1

Summary of published HPLC conditions for RHT determinations.

Column	Sample matrix	Mobile phase	Flow rate	Detection technique	Analysis time	Detection limits	Reference
Waters Spherisorb silica	Human plasma	Acetonitrile-50 mM aqueous sodium dihydrogen phosphate (17 : 83 v/v, pH 3.1)	1.3 mL/min	UV: 200 nm	6 minutes	LOD: 0.2 ng/mL	[26]
						LOQ: 0.5 ng/mL
Inertsil ODS-3V C18	Rat plasma and brain	Ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile 74 : 26 (v/v)	1.0 mL/min	Fluorescence detector, Ex/Em wavelength: 220/293 nm	16 minutes	LOD: not given	[16]
						LOQ: 10 ng/mL
XTerra RP18 C18	Raw material	10 mM sodium-1-heptane sulphonate (pH 3.0) and acetonitrile 72 : 28 (v/v)	1.0 mL/min	UV: 217 nm	13 minutes	LOD: 100 ng/mL	[27]
						LOQ: 300 ng/mL
Monomeric C18	Rat plasma	Acetonitrile and 20 mmol/L phosphate buffer, pH 3.0 (25 : 75)	1.0 mL/min	Fluorescence detector, Ex/Em wavelength:220/293 nm	20 minutes	LOD: not given	[28]
						LOQ: 25 ng/mL
5C18-MS	Capsule	Methanol and water (90 : 10)	1.0 mL/min	UV: 217 nm	Not given	LOD: not given	[29]
						LOQ: 10.9 µg/mL
Kromasil C8	Liposomes	20 mmol·L^−1 phosphate buffer (pH 3.0) and acetonitrile (75 : 25%, v/v)	1.0 mL/min	UV: 210 nm	20 minutes	LOD: not given	[17]
						LOQ: 10 ng/mL
C18	Solid lipid nanoparticles	Acetonitrile and potassium dihydrogen orthophosphate buffer (pH 6.0) (20 : 80 v/v)	1.0 mL/min	UV: 215 nm	Not given	LOD: not given	[14]
						LOQ: 1 µg/mL
ODS C18	Liposomes	Acetonitrile : water (20 mM NaH2PO4·2H2O, 10 mM Na2HPO4·12H2O) (25 : 75, v/v)	1.0 mL/min	UV: 218 nm	Not given	LOD: not given	[15]
						LOQ: not given