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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 1849439, 7 pages
Research Article

Design of Amperometric Biosensors for the Detection of Glucose Prepared by Immobilization of Glucose Oxidase on Conducting (Poly)Thiophene Films

1Department of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, Italy
2Department of Chemical and Geological Sciences, University of Cagliari, Monserrato, 09042 Cagliari, Italy

Correspondence should be addressed to Maria Pilo; ti.ssinu@olipm

Received 12 October 2017; Revised 30 December 2017; Accepted 18 January 2018; Published 1 March 2018

Academic Editor: Marek Trojanowicz

Copyright © 2018 Maria Pilo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Enzyme-based sensors have emerged as important analytical tools with application in diverse fields, and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work, the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2′-bithiophene (2,2′-BT) and 4,4′-bis(2-methyl-3-butyn-2-ol)-2,2′-bithiophene (4,4′-bBT), followed by immobilization of glucose oxidase on the films, is described. N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate (CMC) was used as a condensing agent, and p-benzoquinone (BQ) was used as a redox mediator in solution. The glucose oxidase electrodes with films of 2,2′-BT and 4,4′-bBT were then tested for their ability in detecting glucose from synthetic and real samples (pear, apricot, and peach fruit juices).