Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets

Table 5

Robustness data of the proposed HPLC method.

Parameter		%RSD of standard peak area	%RSD of assay
Column temperature	25°C	0.07	0.15
	30°C (normal)	0.03	0.19
	35°C	0.04	0.2
Wavelength	274 nm	0.06	0.07
	277 nm (normal)	0.03	0.19
	280 nm	0.06	0.17
Mobile phase composition	−5% acetonitrile	0.05	0.20
	Normal	0.03	0.19
	+5% acetonitrile	0.02	0.14
Flow rate	0.8 ml/min	0.04	0.11
	1 ml/min (normal)	0.03	0.19
	1.2 ml/min	0.08	0.23