Journal of Analytical Methods in Chemistry / 2018 / Article / Tab 5

Research Article

Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets

Table 5

Robustness data of the proposed HPLC method.

Parameter%RSD of standard peak area%RSD of assay

Column temperature25°C0.070.15
30°C (normal)0.030.19
35°C0.040.2

Wavelength274 nm0.060.07
277 nm (normal)0.030.19
280 nm0.060.17

Mobile phase composition−5% acetonitrile0.050.20
Normal0.030.19
+5% acetonitrile0.020.14

Flow rate0.8 ml/min0.040.11
1 ml/min (normal)0.030.19
1.2 ml/min0.080.23

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