Research Article

HPLC Method for Quantification of Caffeine and Its Three Major Metabolites in Human Plasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure

Table 1

Final gradient program for HPLC sample analysis.

Time (min)A (%)B (%)Flow rate (ml/min)

09730.7
8.59730.7
129280.7
139730.7

Mobile phase A: 10 mM phosphate buffer, pH 6.8. Mobile phase B: acetonitrile 100%.