HPLC Method for Quantification of Caffeine and Its Three Major Metabolites in Human Plasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure
Table 3
Differences between concentrations of caffeine and metabolites in human plasma or fetal bovine serum spiked sample.
Concentration (µg/mL)
Human plasma mean (CV)
Fetal bovine serum mean (CV)
value
0.5
5.0
30.0
0.5
5.0
30.0
Theb
0.50 (3.4)
5.21 (3.4)
31.49 (4.1)
0.52 (8.8)
5.04 (5.7)
30.57 (7.5)
NS
Par
0.50 (2.6)
5.01 (4.4)
30.92 (7.4)
0.48 (4.0)
5.16 (4.9)
30.62 (6.3)
NS
Theo
0.49 (4.2)
4.98 (4.6)
31.10 (5.2)
0.48 (2.3)
4.95 (4.6)
30.95 (5.5)
NS
Caf
0.52 (6.7)
5.03 (7.0)
32.28 (4.9)
0.50 (8.0)
5.11 (6.6)
31.57 (5.7)
NS
NS: no differences besides groups for each analyte at the same concentration.
