HPLC Method for Quantification of Caffeine and Its Three Major Metabolites in Human Plasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure

Table 4

t-Test analysis for mean comparison between maternal and neonatal caffeine and metabolite concentration. Statistical analysis was done by comparing difference of concentrations mother-child versus zero difference.

Analyte	Mean difference	SE	95% CI difference	Diff (mother-neonate) versus zero
Theobromine	−0.1462	0.087	(−0.327 to 0.035)		NS
Paraxanthine	−0.0352	0.037	(−0.113 to 0.042)		NS
Theophylline	−0.0044	0.028	(−0.064 to 0.055)		NS
Caffeine	−0.0194	0.097	(−0.221 to 0.182)		NS

Caffeine and its metabolites were measured by our HPLC method; CI: confidence interval; Diff: difference; SE: standard error.