Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool

Figure 5

ELISA results for interfering antigens to test the specificity of anti-keratin primary antibody: (a) PBS; (b) negative control; (c) BSA; (d) HSA; (e) ovalbumin; (f) collagen; (g) sericin; (h) fibroin; (i) cotton extraction; (j) hemp extraction; (k) wool extraction; (l) keratin. Each column of the figure represents the mean ± SD (standard deviation) of assays. The dashed line shows the test criterion (the mean OD_PBS plus three times the corresponding standard deviation). If OD_450 nm is above the dashed line, the result is positive, and vice versa.