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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 3158293, 6 pages
Research Article

Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

1School of Biological Sciences and Technology, University of Jinan, Jinan 250022, China
2Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, China

Correspondence should be addressed to Jie Zhou; moc.361@1678eijuohz and Xiao Wang; moc.qq@6546041501

Received 23 August 2017; Revised 20 October 2017; Accepted 26 October 2017; Published 31 January 2018

Academic Editor: Krystyna Pyrzynska

Copyright © 2018 Lei Fang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An efficient method using high-speed counter-current chromatography (HSCCC) coupled with DPPH-HPLC assay has been developed for rapid screening and preparative isolation of antioxidants from ethyl acetate fraction of Alpinia officinarum Hance. Target-guided by DPPH-HPLC assay, two antioxidants, galangin and kaempferide, were targeted and further separated with purities of 99.3% and 98.5% by HSCCC using petroleum ether–ethyl acetate–methanol–water (0.8 : 1 : 1 : 0.8, v/v) as the solvent system. The antioxidant activities of galangin and kaempferide were further evaluated by measuring their inhibiting effects on superoxide anion radical, hydroxyl radical, and hydrogen peroxide in different luminol chemiluminescence (CL) systems. As a result, galangin and kaempferide both showed potent antioxidant activities. Results of the present study indicated that the combinative method by offline coupling DPPH-HPLC and HSCCC could be widely applied for rapid screening and isolation of antioxidants from complex TCM extract.