Research Article
Determination of Curdlan Oligosaccharides with High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection
Table 1
Linearity of the log10 RT versus DPs (2–6) of COS separated by HPAEC on a CarboPacPA-100 column.
| Entry | Mobile phase (A : B : C)a | Flow rate (mL/min) | Linear equationb | R2 |
| 1 | 40 : 40 : 20 | 0.5 | y = 0.2183x + 0.2208 | 0.9964 | 2 | 40 : 40 : 20 | 0.8 | y = 0.2204x + 0.0137 | 0.9963 | 3 | 40 : 40 : 20 | 1.0 | y = 0.2199x − 0.0811 | 0.9964 | 4 | 30 : 50 : 20 | 0.8 | y = 0.2408x − 0.0104 | 0.9964 | 5 | 50 : 30 : 20 | 0.8 | y = 0.1892x + 0.0525 | 0.9962 | 6 | 35 : 40 : 25 | 0.8 | y = 0.1597x + 0.0839 | 0.9942 | 7 | 45 : 40 : 15 | 0.8 | y = 0.2949x − 0.0520 | 0.9981 |
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aA: water; B: 0.2 M sodium hydroxide; C: 0.5 M sodium acetate; by and x refer to the log10 RT and DP, respectively.
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