Research Article

Determination of Curdlan Oligosaccharides with High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

Table 1

Linearity of the log10 RT versus DPs (2–6) of COS separated by HPAEC on a CarboPacPA-100 column.

EntryMobile phase (A : B : C)aFlow rate (mL/min)Linear equationbR2

140 : 40 : 200.5y = 0.2183x + 0.22080.9964
240 : 40 : 200.8y = 0.2204x + 0.01370.9963
340 : 40 : 201.0y = 0.2199x − 0.08110.9964
430 : 50 : 200.8y = 0.2408x − 0.01040.9964
550 : 30 : 200.8y = 0.1892x + 0.05250.9962
635 : 40 : 250.8y = 0.1597x + 0.08390.9942
745 : 40 : 150.8y = 0.2949x − 0.05200.9981

aA: water; B: 0.2 M sodium hydroxide; C: 0.5 M sodium acetate; by and x refer to the log10 RT and DP, respectively.