DNA Nanotweezers with Hydrolytic Activity for Enzyme-Free and Sensitive Detection of Fusion Gene via Logic Operation

Figure 2

Optimization of experimental conditions. (a) Time-dependent fluorescence changes from 20, 30, 40, 50, 60, to 70 min. (b) DNAzyme cleavage activity on varied reaction temperature at 25, 30 35, 40, 45, and 50°C. (c) The effect of bases number on hinge region on signal to noise by setting at 3, 4, 5, 6, and 7 nucleotides.

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(b)

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