Investigation of Interactions between Thrombin and Ten Phenolic Compounds by Affinity Capillary Electrophoresis and Molecular Docking

Table 1

Interactions of ten investigated compounds with thrombin evaluated by ACE.

Compounds	Molecule weight	pKa	Detection wavelength (nm)	Regression equation of and	Binding constant (mL/U)
p-Hydroxybenzoic acid	138.12	4.57 ± 0.10	250	—	—
Protocatechuic acid	154.12	4.45 ± 0.10	250	—	—
Vanillic acid	168.15	4.45 ± 0.10	250	—	—
Gallic acid	170.12	4.33 ± 0.10	250	y = −0.184x + 0.105 (R^2 = 0.95)	0.184
Naringenin	272.25	7.52 ± 0.40	280	y = −0.238x + 0.117 (R^2 = 0.99)	0.238
Apigenin	270.24	6.53 ± 0.40	280	y = −0.302x + 0.147 (R^2 = 0.99)	0.302
Baicalein	270.24	6.31 ± 0.40	280	y = −0.508x + 0.093 (R^2 = 0.94)	0.508
Catechin	290.27	9.54 ± 0.10	280	y = −0.353x + 0.080 (R^2 = 0.98)	0.353
Epicatechin	290.27	9.54 ± 0.10	280	y = −0.297x + 0.043 (R^2 = 0.99)	0.297
Dihydroquercetin	304.25	7.39 ± 0.60	250	y = −0.389x + 0.075 (R^2 = 0.99)	0.389