Research Article
Pharmacokinetics of Eight Flavonoids in Rats Assayed by UPLC-MS/MS after Oral Administration of Drynariae rhizoma Extract
Table 5
Stability evaluation results (
).
| Analytes | Spiked (ng/ml) | Autosampler (4°C, 36 h) | Long-term (−20°C, 30 days) | Freeze-thaw (−20°C-room temperature) | Measured | RE (%) | Measured | RE (%) | Measured | RE (%) |
| Neoeriocitrin | 40.4 | 40.29 ± 1.99 | −1.00 | 40.5 ± 2.82 | −1.21 | 39.8 ± 1.92 | −2.92 | 202 | 198.79 ± 11.22 | 2.58 | 206.55 ± 12.4 | 1.72 | 205.16 ± 23.24 | 1.36 | 2020 | 2001.91 ± 21.3 | −0.90 | 2012.25 ± 27.81 | −0.37 | 2032.19 ± 45.28 | 0.61 | Luteolin-7-O-β-D-glucoside | 5.04 | 5.02 ± 0.18 | −3.12 | 4.99 ± 0.07 | −2.34 | 5.01 ± 0.21 | −1.26 | 50.4 | 49.44 ± 1.55 | −1.38 | 49.37 ± 3.07 | −3.64 | 49.55 ± 8.55 | −2.45 | 504 | 494.39 ± 10.55 | −1.90 | 493.07 ± 13.07 | −2.16 | 491.55 ± 18.55 | −2.47 | Astragalin | 5.1 | 5.02 ± 0.18 | −1.63 | 4.99 ± 0.07 | −2.18 | 5.01 ± 0.21 | −1.79 | 102 | 101.10 ± 4.29 | −2.79 | 99.7 ± 3.76 | −4.13 | 102.04 ± 4 | −1.88 | 1020 | 1006.41 ± 30.28 | 3.56 | 1002.46 ± 35.15 | 1.22 | 1000.65 ± 34.59 | 1.04 | Naringin | 10.2 | 10.5 ± 0.14 | −4.02 | 10.3 ± 0.09 | −0.27 | 9.9 ± 0.18 | −1.11 | 408 | 401.01 ± 8.13 | −0.24 | 400.90 ± 9.65 | −0.51 | 403.52 ± 11.42 | −0.11 | 6120 | 6008.15 ± 38.45 | −5.66 | 6010.28 ± 22.59 | −3.22 | 6009.12 ± 43.76 | −4.99 | Eriodictyol | 1.02 | 0.96 ± 0.06 | −1.30 | 1.06 ± 0.06 | −4.22 | 0.99 ± 0.07 | −2.84 | 40.8 | 38.83 ± 2.5 | −0.44 | 39.87 ± 1.83 | 2.28 | 39.00 ± 2.32 | −2.64 | 816 | 834.09 ± 23.38 | −0.70 | 837.06 ± 26.59 | −0.34 | 824.21 ± 18.77 | −1.88 | Luteolin | 10.1 | 10.2 ± 0.04 | 3.54 | 9.97 ± 0.09 | −0.13 | 9.96 ± 0.1 | −1.04 | 40.4 | 37.85 ± 1.42 | 0.94 | 38.08 ± 1.5 | 1.55 | 39.39 ± 1.9 | −0.29 | 1010 | 1000.91 ± 21.3 | −1.91 | 1006.25 ± 27.81 | −0.25 | 1016.19 ± 45.28 | 0.64 | Naringenin | 5.2 | 5.1 ± 0.06 | 0.92 | 5.1 ± 0.11 | 0.89 | 5.08 ± 0.11 | 0.66 | 41.6 | 41.50 ± 3.05 | −1.20 | 41.33 ± 3.09 | −1.60 | 41.13 ± 3.11 | −2.06 | 208 | 207.10 ± 4.29 | −4.79 | 208.70 ± 5.76 | −8.13 | 204.04 ± 8 | −3.76 | Kaempferol | 1.06 | 1.05 ± 0.14 | −3.02 | 1.03 ± 0.09 | −0.49 | 1.09 ± 0.18 | −1.71 | 42.4 | 40.59 ± 1.39 | −1.07 | 41.15 ± 2.32 | −1.34 | 41.8 ± 1.58 | −2.67 | 424 | 419.01 ± 7.42 | −0.44 | 427.9 ± 3.74 | −0.46 | 419.52 ± 8.323 | −0.31 |
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