Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Table 1

Chromatographic parameters for separation of polyphenols^a.

Parameter	GA	VA	CA	FA	p-COA
tm (min)	1.30	1.30	1.30	1.30	1.30
tr (min)	1.58	5.00	7.10	9.70	13.7
t’r (min)	0.28	3.72	5.80	8.40	12.4
k	0.22	2.86	4.49	6.50	9.60
α	1.00	13.3	20.9	30.0	44.4

^aExperimental conditions are as shown in Figure 4; GA, gallic acid; VA, vanillic acid; CA, caffeic acid; FA, ferulic acid; p-COA, p-coumaric acid.