Research Article
Development and Validation of a Versatile UPLC-PDA Method for Simultaneous Determination of Paracetamol, Tizanidine, Aceclofenac, and Nimesulide in Their New Combinations
Figure 2
Typical UPLC chromatogram for simultaneous separation of PAR, TIZ, ACF, and NIM. Chromatographic conditions are as follows: ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 m); mobile phase consisting of acetonitrile : 20 mM phosphate buffer adjusted to pH 7.0 using dipotassium hydrogen phosphate (40 : 60 /); flow rate 1.25 mL·min−1; injection volume 2.0 L; and PDA detection at 305 nm.