Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC

<div>Comparison of the relative contents of the 14 LSW components in the ethanolic and aqueous extraction liquors of LSW. The peaks 1, 10, 11, 12, 13, and 14 correspond to 5-hydroxytryptamine, bufotalin, cinobufotalin, bufalin, cinobufagin, and resibufogenin, respectively.</div>

Journal of Analytical Methods in Chemistry

fig2

Figure 2

Figure 2: Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC 

Figure 2 | Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC 