Research Article
Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC
Table 1
Method validation through the detection of 6 different compounds in the ethanol extraction of LSW.
| Peak number | Compound | Intraday RSD (%) | Interday RSD (%) | Recovery (%) | LOD (ng/mL) | LOQ (ng/mL) |
| 1 | 5-Hydroxytryptamine | 0.31 | 0.38 | 101.2 | 8.22 | 27.4 | 10 | Bufotalin | 0.84 | 1.30 | 103.2 | 5.28 | 17.6 | 11 | Cinobufotalin | 0.69 | 1.42 | 94.5 | 7.47 | 24.9 | 12 | Bufalin | 0.13 | 0.70 | 106.9 | 11.26 | 37.7 | 13 | Cinobufagin | 0.16 | 0.65 | 104.3 | 1.11 | 3.70 | 14 | Resibufogenin | 0.15 | 0.31 | 101.1 | 7.81 | 26.0 |
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