Research Article

Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC

Table 1

Method validation through the detection of 6 different compounds in the ethanol extraction of LSW.

Peak numberCompoundIntraday RSD (%)Interday RSD (%)Recovery (%)LOD (ng/mL)LOQ (ng/mL)

15-Hydroxytryptamine0.310.38101.28.2227.4
10Bufotalin0.841.30103.25.2817.6
11Cinobufotalin0.691.4294.57.4724.9
12Bufalin0.130.70106.911.2637.7
13Cinobufagin0.160.65104.31.113.70
14Resibufogenin0.150.31101.17.8126.0