Research Article

Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Table 1

Red-white conditional formatting table between three variables (matrix, base, and tagging reagent) and the MALDI R/N ratio of maltohexaose or its Girard derivatives. ND = not detected. Color scale: deeper red highlighted cells correspond to higher R/N ratio.

DerivatizationMatrixBase
AnMITMGTEAPyr

Girard’s TDHB2455ND63224
CHCA3353,9701,3174,97262
pCANDNDNDNDND

Girard’s PDHB99NDND369
CHCA2,8234,423152,84243
pCAND4NDNDND

NonderivatizationDHB18NDNDND248
CHCA6214,421104,974354
pCANDNDNDNDND