Determination of Tobramycin in M₉ Medium by LC-MS/MS: Signal Enhancement by Trichloroacetic Acid

Figure 3

Impact of mobile phase solvents and sample solvents on peak shape, retention time, and signal intensity of TBM. Sample solvents: water (dash line) and 2% TCA (solid line). Mobile phase solvents: 10 mM NH₄FA (pH 4.0)-0.1% FA in MeCN (a) and 20 mM NH₄FA 0.14% TFA-0.1% TFA in MeCN (b).

(a)

(b)