Research Article
Determination of Tobramycin in M9 Medium by LC-MS/MS: Signal Enhancement by Trichloroacetic Acid
| | Concentration (ng/ml) | TBM peak area (×104) | IS peak area (×104) | Ratio | Matrix effect | | Water | M9 | Water | M9 | Water | M9 | TBM | IS | Ratio |
| | Low (120) | 3.60 ± 0.12 | 3.83 ± 0.13 | 8.26 ± 0.49 | 8.72 ± 0.53 | 0.436 | 0.439 | 106 | 106 | 101 | | Medium (1500) | 18.1 ± 1.0 | 20.0 ± 1.4 | 8.80 ± 0.77 | 9.30 ± 0.91 | 2.06 | 2.15 | 110 | 106 | 104 | | High (17000) | 378 ± 5 | 413 ± 18 | 13.7 ± 0.5 | 14.7 ± 0.7 | 27.6 | 28.1 | 109 | 107 | 102 |
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Data represent the mean peak area (±SD) from triplicate analysis.
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