One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS
Table 5
Intraday and interday precision and accuracy of the analytes in human plasma ().
Analyte
Nominal concentration (ng/mL)
Intraday ()
Interday ()
Measured concentration (mean ± SD, ng/mL)
Precision (%RSD)
Accuracy (%RE)
Measured concentration (mean ± SD, ng/mL)
Precision (%RSD)
Accuracy (%RE)
Paclitaxel
50.0
47.78 ± 1.26
2.6
−4.4
47.79 ± 1.69
3.5
−4.4
250.0
250.06 ± 11.71
4.7
0.0
260.39 ± 11.21
4.3
4.2
1250.0
1309.21 ± 46.73
3.6
4.7
1278.69 ± 78.67
6.2
2.3
Docetaxel
20.0
18.56 ± 0.40
2.2
−7.2
18.76 ± 0.44
2.3
−6.2
100.0
96.10 ± 3.69
3.8
−3.9
101.56 ± 5.11
5.0
1.6
500.0
538.07 ± 21.68
4.0
7.6
518.53 ± 6.09
1.2
3.7
SN-38
20.0
17.56 ± 0.92
5.2
−12.2
16.92 ± 0.42
2.5
−10.4
100.0
103.26 ± 8.86
8.6
3.3
93.16 ± 1.86
2.0
−6.8
500.0
546.59 ± 33.33
6.1
9.3
504.15 ± 50.82
10.1
0.8
Vinorelbine
200.0
173.52 ± 1.84
1.1
−13.2
171.59 ± 2.36
1.4
−14.2
1000.0
1005.74 ± 5.48
0.5
0.6
992.62 ± 4.10
0.4
−0.7
5000.0
5364.49 ± 2.62
0.1
7.3
5244.73 ± 2.17
0.1
4.9
Pemetrexed
200.0
185.20 ± 13.86
7.5
−7.4
181.32 ± 14.97
8.3
−9.3
1000.0
951.42 ± 72.67
7.6
−4.9
955.55 ± 39.65
4.1
−4.4
5000.0
4708.39 ± 445.31
9.5
−5.8
5230.11 ± 168.09
3.2
4.6
Vinblastine
50.0
47.51 ± 0.95
5.4
−5.0
47.08 ± 1.99
11.7
−5.8
500.0
497.29 ± 9.82
5.0
−0.5
492.92 ± 5.17
2.7
−1.4
5000.0
5226.19 ± 2.35
0.1
4.5
4990.19 ± 3.67
0.1
−0.2
Irinotecan
50.0
49.89 ± 1.07
5.4
−0.2
47.66 ± 0.94
5.3
−4.7
500.0
497.83 ± 8.38
4.2
−0.4
495.69 ± 6.01
3.1
−0.9
5000.0
5379.31 ± 178.38
3.3
7.6
5006.16 ± 196.44
3.9
0.1
Cyclophosphamide
5.0
5.00 ± 0.21
10.4
0.0
4.95 ± 0.23
11.7
−1.0
50.0
49.27 ± 0.69
3.4
−1.5
48.39 ± 0.31
1.6
−3.2
500.0
538.97 ± 5.80
1.2
7.8
525.12 ± 9.37
1.9
5.0
Ifosfamide
5.0
4.90 ± 0.10
5.1
−2.0
4.91 ± 0.08
4.0
−1.8
50.0
49.04 ± 0.51
2.7
−1.9
49.16 ± 0.71
3.7
−1.7
500.0
526.72 ± 10.80
2.1
5.3
516.36 ± 12.78
2.5
3.3
Carboplatin
100.0
98.57 ± 2.59
2.6
−1.4
103.05 ± 12.69
12.3
3.1
500.0
479.21 ± 5.69
1.2
−4.2
477.25 ± 11.46
2.4
−4.6
2500.0
2640.10 ± 40.11
1.5
5.6
2537.97 ± 61.41
2.4
1.5
Etoposide
100.0
99.71 ± 2.56
2.6
−0.3
98.31 ± 2.00
2.0
−1.7
500.0
482.60 ± 16.72
3.5
−3.5
465.72 ± 14.92
3.2
−6.9
2500.0
2711.88 ± 59.84
2.2
8.5
2633.31 ± 72.39
2.7
5.3
Gemcitabine
100.0
101.86 ± 7.55
7.4
1.9
86.53 ± 5.64
6.5
−13.5
500.0
526.06 ± 47.53
9.0
5.2
477.32 ± 33.61
7.0
−4.5
2500.0
2546.58 ± 100.92
4.0
1.9
2402.05 ± 106.44
4.4
−3.9
aRE is calculated as (mean measured concentration/spiked concentration − 1) × 100%.
