Research Article
Development and Validation of an LC-MS/MS Method and Comparison with a GC-MS Method to Measure Phenytoin in Human Brain Dialysate, Blood, and Saliva
Table 1
Settings of the HPLC program.
| Time (minutes) | Pump 1 (main column (MC)) | Pumps 2 and 3 (trapping column (TC)) | %B | Flow (µL/min) | Comments | %B | Flow (µL/min) | Flow pump 5 (H2O + 0.1% HCOOH) (µL/min) | Switching valve |
| 0 | 35 | 500 | Start MS and pumps | 50 | 300 | 800 | TC → waste, MC → MS (loading) | 0.5 | 35 | Start gradient | 50 | 300 | 800 | TC → MC → MS (eluting) | 0.6 | ↓ | | 50 | 20 | 20 | | 1 | 97.5 | | | 2 | 97.5 | | TC → waste, MC → MS | 2.5 | 35 | | 50 | 300 | 800 | Reequilibration |
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