Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms

Figure 1

Original calorimetric recording of the apparent heat capacity of isoforms β-HaH (a) and α_D+N-HaH (b) as a function of the temperature (line B); reheating scan (line C); and baseline obtained with buffer in both cells of the calorimeter (line A). The experimental curves shown refer to β-HaH (2.88 mg·ml⁻¹) and α_D+N-HaH (3 mg·ml⁻¹) in buffer 20 mM HEPES, containing 100 mM NaCl, 5 mM CaCl₂, and 5 mM MgCl₂ (pH 7.06 at 80°C), at scan rate of 1.0°C min⁻¹.

(a)

(b)